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Bosphore Malaria Genotyping Kit v1

Bosphore® Malaria Genotyping Detection Kit v1 is, a Real-Time PCR-based in vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore® Malaria Genotyping Detection Kit v1 detects and discriminates Plasmodium species in human body fluids such as serum, plasma, saliva, urine etc. Fluorescence detection is accomplished using FAM, HEX and Cy5 filters in two PCR tubes.

 

In the first PCR tube PCR Master Mix 1, Plasmodium falciparum genome is amplified and fluorescence detection is accomplished using the FAM filter and Plasmodium malariae genome is amplified and fluorescence detection is accomplished using the HEX filter.

 

In the second PCR tube PCR Master Mix 2, Plasmodium vivax genome is amplified and fluorescence detection is accomplished using the FAM filter and Plasmodium ovale genome is amplified and fluorescence detection is accomplished using the HEX filter.

 

An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter in both PCR tubes with PCR Master Mix 1 and 2. The internal control is added during PCR step.