Bosphore STDs Panel Kit v1

Bosphore® STD Panel Kit v1 is a Real-Time PCR-based in vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore® STD Panel Kit v1 detects and characterizes Ureaplasma urealyticumUreaplasma parvumMycoplasma hominisNeisseria gonorrheaTrichomonas vaginalis and Mycoplasma genitalium in human biological samples. Fluorescence detection is accomplished using the FAM, HEX, Texas RED and Cy5 filters.

 

In the first PCR tube with PCR Master Mix 1, Ureaplasma urealyticum genome is amplified and fluorescence detection is accomplished using the FAM filter, Ureaplasma parvum genome is amplified and  fluorescence detection is accomplished using the HEX filter and Mycoplasma Hominis genome is amplified and fluorescence detection is accomplished using the Texas RED filter.

 

In the second PCR tube with PCR Master Mix 2, Neisseria gonorrhea genome is amplified and fluorescence detection is accomplished using the FAM filter, Trichomonas vaginalis genome is amplified and fluorescence detection is accomplished using the HEX filter and Mycoplasma genitalium genome is amplified and fluorescence detection is accomplished using the Texas RED filter.


An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter in both PCR tubes. The internal control can be added either during DNA extraction or PCR step.