We use cookies to improve your experience on our site. By using our site, you accept cookies.  Allow

Bosphore HPV Genotyping High Risk Kit v4 (Fast Extraction)

Bosphore® HPV Genotyping High Risk Kit v4 (Fast Extraction) is a Real-Time PCR-based in vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore® HPV Genotyping High Risk Kit v4 detects and characterizes the genotype of Human Papilloma Virus in human biological samples, encompassing high risk group of HPV genotypes (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68). Fluorescence detection is accomplished using the FAM, HEX, Texas RED and Cy5 filters.

 

  • The genotypes 16, 18, 31 and internal control are detected in PCR Master Mix 1;
  • The genotypes 51, 56, 58 and internal control are detected in PCR Master Mix 2,
  • The genotypes 45, 35, 59 and internal control are detected in PCR Master Mix 3,
  • The genotypes, 39, 66, 68 and internal control are detected in PCR Master Mix 4 and
  • The genotypes 33, 52 and internal control are detected in PCR Master Mix 5 with using labeled probes. The five PCR master mixes function in five separate wells/reactions.

 

An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter. The internal control is added into the sample at the PCR step. Only a single incubation with the EX-Tract DNA solution is enough to prepare the samples for qPCR. There is no need to apply an external DNA isolation.