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Bosphore CNS Mini Panel Kit v2

Bosphore® CNS Mini Panel Kit v2 is a Real-Time PCR-based in vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore® CNS Mini Panel Kit v2 detects and characterizes Herpes simplex virus 1, Herpes simplex virus 2, Varicella-Zoster virus, Parvovirus B19, Human Herpesvirus 6 (HHV-6), Human Herpesvirus 7 (HHV-7), Enterovirus and Parechovirus DNA in human biological samples. Fluorescense detection is accomplished using the FAM, HEX, Texas Red and Cy5 Filters.

 

In the first PCR tube with PCR Master Mix 1, Herpes Simplex Virus 1 genome is amplified and fluorescence detection is accomplished using the FAM filter, Herpes Simplex Virus 2 genome is amplified and fluorescence detection is accomplished using the Cy5 filter, Varicella-Zoster Virus genome is amplified and fluorescence detection is accomplished using HEX filter.

 

In the second PCR tube with PCR Master Mix 2, Human Herpesvirus 6 (HHV-6) genome is amplified and fluorescence detection is accomplished using FAM Filter, Parvovirus B19 genome is amplified and fluorescence detection is accomplished using HEX filter, Human Herpesvirus 7 (HHV-7) genome is amplified and fluorescence detection is accomplished using Cy5 filter.

 

In the third PCR tube with PCR Master Mix 3, Enterovirus is amplified and fluorescence detection is accomplished using the FAM filter and Parechovirus is amplified and fluorescence detection is accomplished using the HEX filter.

An internal control has been integrated into the kit in order to check DNA extraction and PCR inhibition. The amplification data of the internal control is detected with Texas Red Filter. The internal control can be added either during DNA extraction or PCR step.