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Bosphore Norovirus Detection Kit v1

Bosphore® Norovirus Detection Kit v2 detects norovirus GI and GII RNA from fruit and vegetable samples, sea food, meat, poultry and dairy products. Fluorescence detection is accomplished using the FAM, HEX and Cy5 filters. Norovirus genogroup GI genome is amplified and fluorescence detection is accomplished using FAM filter and norovirus genogroup GII genome is amplified and fluorescence detection is accomplished using HEX filter. Analytical detection limit for Bosphore® Norovirus Detection Kit v2 was found to be 13 copies/reaction for Norovirus GI and 20 copies/reaction for Norovirus GII.

 

A process control has been integrated into the kit to control extraction efficiency. Amplification data of the process control is detected with the Cy5 filter. Also, positive control (external control-EC) has been integrated to the kit to control RT-PCR inhibition and to act as an RT-PCR positive control.