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Bosphore FII-FVL Detection Kit v1

Bosphore® FII-FVL Detection Kit v1 detects Factor II mutation in the 3’UTR region of FII gene; namely G20210A (Prothrombin mutation/ a change of guanine to adenine) and Factor V Leiden mutations; namely G1691A (a change of glutamic acid to arginine) in human biological samples. 

 

Wild type FII gene is amplified and fluorescence detection is accomplished using Cy5 filter. FII mutation is amplified and fluorescence detection is accomplished using Texas RED filter. Wild type F5 allele is amplified and fluorescence detection is accomplished using the HEX filter. Mutant F5 allele is amplified and fluorescence detection is accomplished using the FAM filter.