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Bosphore Thrombophilia Panel Kit v2 (Fast Extraction)

Bosphore® Thrombophilia Panel Kit v2 (Fast Extraction) detects Factor II mutation in the 3’UTR region of FII gene; namely G20210A (Prothrombin mutation/a change of guanine to adenine) and Factor V Leiden mutations; namely G1691A (a change of glutamic acid to arginine), MTHFR C677T mutation (a change of cytosine nucleotide to thymine at position 677), and MTHFR  A1298C mutation (a change of adenine nucleotide to cytosine at position 1298)  in human biological samples. 


In the first PCR tube with PCR Master Mix 1, Wild type FII gene is amplified and fluorescence detection is accomplished using Cy5 filter. FII mutation is amplified and fluorescence detection is accomplished using Texas RED filter. Wild type F5 allele is amplified and fluorescence detection is accomplished using the HEX filter. Mutant F5 allele is amplified and fluorescence detection is accomplished using the FAM filter.


In the second PCR tube with PCR Master Mix 2, Wild type MTHFR A1298C gene is amplified and fluorescence detection is accomplished using Cy5 filter. Mutant MTHFR A1298C gene is amplified and fluorescence detection is accomplished using Texas RED filter. Wild type MTHFR C677T gene is amplified and fluorescence detection is accomplished using the HEX filter. Mutant MTHFR C677T gene is amplified and fluorescence detection is accomplished using the FAM filter.