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Bosphore Novel Coronavirus (2019-nCoV) Detection Kit v2

Bosphore Novel Coronavirus (2019-nCoV) Detection Kit v2 is a Real-Time PCR-based in vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. 

 

Bosphore® Novel Coronavirus (2019-nCoV) Detection Kit v2 detects and characterizes 2019-nCoV (which is later renamed officially as Severe acute respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by WHO) in human respiratory samples including nasopharyngeal, oropharyngeal swabs and sputum. Fluorescence detection is accomplished using FAM, HEX and Cy5 filters. 2019-nCoV is detected by two regions of the virus in two separate reactions: E gene is used for screening purpose, where 2019-nCoV and also the closely related coronaviruses are detected, and the orf1ab target region is used to discriminate 2019-nCoV specifically. In PCR Master Mix, 2019-nCoV target region is amplified and fluorescence detection is accomplished using FAM filter, 2019-nCoV screening region (E gene) is amplified and fluorescence detection is accomplished using the Cy5 filter.


An internal control has been integrated into the kit in order to check RNA extraction, PCR inhibition and application errors. The amplification data of the internal control is detected with HEX filter.

 

The internal control can be added either during RNA extraction or PCR step.

 

In order to tackle the existing global shortage on extraction kits under these extra-ordinary circumstances, we have developed an alternative, fast-extraction, which does not require a separate extraction but only a pre-treatment that takes less than 10 minutes. This method helps the institutions and medical workers save precious time by eliminating the need for extraction for diagnosis. Bosphore EX-Tract Dry Swab RNA Solution has been optimized to perform only with Bosphore Novel Coronavirus (2019-nCoV) Detection Kits.

 

FAM

HEX

Cy5

Orf1ab

Internal Control

E gene