Magnesia Tissue Genomic DNA Extraction Kit

401 Magnesia Genomic DNA Tissue Kit

 

Magnesia®Genomic DNA Tissue Kit is designed for purification of total DNA (including genomic, mitochondrial and viral DNA) from a variety of animal tissues or cells by using Magnesia®16 auto-extraction instrument. The provided Filter Column Set can filtrate hard tissue sample or swab sample to prevent tissue residues to obstruct pipette syringe during the process of Magnesia®16. The method uses pre-filled cartridge contains proteinase K and a chaotropic salt, guanidine hydrochloride to lysis cells and degrade protein. DNA in chaotropic salt binds to cellulose coated magnetic beads. After washing off the contaminants, the purified DNA is eluted by low salt elution buffer or water. Purified DNA of approximately 20-30 kb in length is suitable for PCR or other enzymatic reactions.

 


Specifications

Magnesia®Genomic DNA Tissue Kit is based on magnetic extraction method using the cellulose coated magnetic beads. Genomic DNA can be easily obtained in high purity within a short time by simply introducing the cartridge, choosing the required protocol and starting the Magnesia®16 System.

 


Applications

For general laboratory use.  Magnetic-particle technology is used to purify the genomic DNA from a variety animal tissues, paraffin-embedded tissue, swab and blood. The purified genomic DNA can be directly used for downstream application such as quantitative PCR, restriction enzyme digestion and southern blotting and RAPD/AFLP.

 

 

Cartridge Design

 

Magnesia®reagent cartridges come pre-sealed incorporating all the reagents needed for purification processes. No additional reagents are needed and no handling is necessary beyond placing the cartridges inside the instrument. Pre-filled and sealed buffer cartridge plus automated piercing step whole eliminate possible contaminations or buffer spouts.

 
Patented Heating Well and Separation Well located in the cartridge may provide strong circulation- force for Binding/Washing steps and thus give high purity within final elutant.

 

Each cartridge has 14 positions available with 10 sealed wells and two optional heating positions. Special program code designs required protocols.  Magnesia reagent cartridge has its own program code depending on the application.

 

 

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