Montania 484 Real-Time PCR Instrument

It is well known that the accuracy of the thermal cycles and achieving temperature uniformity between tubes, so that all samples undergo the same thermal conditions, are prerequisite for a reliable PCR reaction.

Key to a precise and sensitive quantification analysis lies in the error-free detection and analysis of low levels of fluorescence.

 In order to realize these fundamentals, Montania® 484 is equipped with substantial structural features to meet the requirements and expectations of both diagnostic and research fields.      

Temperature distribution of +/- 0.1 °C throughout the whole thermal block, a LED light which is capable of exciting with constant intensity during its practically lifelong use, fiber optic translation of every emission read-out and a PMT module system that enables exquisite measurement via physical amplification of each photon’s energy.

And the outstanding software that allows manual optimization in addition to its automated analysis option, completes the picture.


The result is;

* No background noise, no need for calibration;

* Inter-instrument, and inter-well variation below 0.4 CT;

* A superior performance where standard curve correlation coefficients of 0.999 are regarded as ordinary, in addition to reliable, sensitive and reproducible studies;

* A perfect colleague that undertakes all the cumbersome work, with its automated configuration and optional barcode reader system…


A graph demonstrating the overlapping amplification curves of 16  HBV DNA standards (1X104 IU/ml) and 4 negative controls that were tested in four separate instruments.


Sample capacity48
Light sourceLED
Temperature range4-99ºC
Max ramp rate4.5ºC/sec
Temperature accuracy+/- 0.1ºC
Temperature uniformity+/- 0.1ºC
Sample size15-100 µl
Filter pairs/Wavelengths 4 (470/510 - 530/565 - 580/620 - 630/665)
DyesFAM/SYBR Green – VIC/HEX/JOE/TET/TAMRA –  ROX/Texas Red – Cy5
Dimensions (cm)38x52x25
Weight18 kg